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  Protein-interfaced Biosensors

 

 
Increasing concern regarding the microbiological safety of food, water, dairy products, industrial waste, and pharmaceutical preparations has provided an urgency for detection methods that are fast, sensitive, specific, reliable, and quantitative for quality assurance in order to prevent infections and epidemics. There are a large number of detection methods for microorganisms, including immunomagnetic separation and flow cytometry, flexural plate wave, quartz crystal, and surface acoustic wave.

The green fluorescent protein is used as a popular choice for development of reporter-biosensors to detect various environmentally hazardous compounds. The approach that couples site-directed mutagenesis of green fluorescent protein and rational computational design generates a fluorescence-based biosensor for endotoxin and gram-negative bacteria.

 

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