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Increasing concern regarding the
microbiological safety of food, water, dairy products, industrial waste, and
pharmaceutical preparations has provided an urgency for detection methods that
are fast, sensitive, specific, reliable, and quantitative for quality assurance
in order to prevent infections and epidemics. There are a large number of
detection methods for microorganisms, including immunomagnetic separation and
flow cytometry, flexural plate wave, quartz crystal, and surface acoustic wave.
The green fluorescent protein is
used as a popular choice for development of reporter-biosensors to detect
various environmentally hazardous compounds. The approach that couples
site-directed mutagenesis of green fluorescent protein and rational
computational design generates a fluorescence-based biosensor for endotoxin and
gram-negative bacteria. |